Article

Two-Step Approach for Cleaning & Disinfection of Bacillus Cereus Biofilm

Biofilms can be a persistent problem for those cleaning production equipment in GMP cleanroom facilities. Our STERIS experts have shown however that a combination of an effective alkaline cleaning detergent followed by a sporicide is an effective means to address even the most highly resistant biofilm cell populations such as B. cereus.
November 9, 2018
Daniel Klein
Paul Lopolito

Key Takeaways:

  • Bacillus cereus biofilms resist disinfection due to spores and protective matrix.
  • Using alkaline detergent first, then a sporicide improves biofilm reduction.
  • Pre-cleaning disrupts biofilm, enhancing sporicidal treatment effectiveness.
  • Bacillus cereus biofilms are tougher than spores or non-spore biofilms.
  • This validated method helps control biofilms in GMP-regulated cleanrooms. 

“Two-step approach for cleaning and disinfection of Bacillus cereus biofilm” written by our STERIS experts, Amanda Deal, Rebecca Hostettler, Dan Klein and Paul Lopolito, has been published in this month’s edition of Cleanroom Technology (October 2017).  

Biofilms can be a persistent problem for those cleaning production equipment in GMP cleanroom facilities. Our STERIS experts have shown however that a combination of an effective alkaline cleaning detergent followed by a sporicide is an effective means to address even the most highly resistant biofilm cell populations such as B. cereus.

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